New degradative routes of 5-hydroxytryptophan and serotonin by intestinal tryptophan 2, 3-dioxygenase
F Hirata, O Hayaishi - Biochemical and biophysical research …, 1972 - Elsevier
F Hirata, O Hayaishi
Biochemical and biophysical research communications, 1972•ElsevierA highly purified preparation of tryptophan 2, 3-dioxygenase from rabbit intestine was found
to catalyze the oxygenative ring cleavage of 5-hydroxytryptophan and serotonin. The
products of these enzymic reactions were susceptible to the action of formamidase, as a
consequence of which 5-hydroxykynurenine and 5-hydroxykynurenamine were isolated
from the reaction mixtures and identified. The initial products were presumed, therefore, to
be 5-hydroxyformylkynurenine and 5-hydroxyformylkynurenamine, respectively. Several …
to catalyze the oxygenative ring cleavage of 5-hydroxytryptophan and serotonin. The
products of these enzymic reactions were susceptible to the action of formamidase, as a
consequence of which 5-hydroxykynurenine and 5-hydroxykynurenamine were isolated
from the reaction mixtures and identified. The initial products were presumed, therefore, to
be 5-hydroxyformylkynurenine and 5-hydroxyformylkynurenamine, respectively. Several …
Abstract
A highly purified preparation of tryptophan 2,3-dioxygenase from rabbit intestine was found to catalyze the oxygenative ring cleavage of 5-hydroxytryptophan and serotonin. The products of these enzymic reactions were susceptible to the action of formamidase, as a consequence of which 5-hydroxykynurenine and 5-hydroxykynurenamine were isolated from the reaction mixtures and identified. The initial products were presumed, therefore, to be 5-hydroxyformylkynurenine and 5-hydroxyformylkynurenamine, respectively. Several lines of evidence indicated that the cleavage of tryptophan, 5-hydroxytryptophan and serotonin occurred by the action of a single protein, namely intestinal tryptophan 2,3-dioxygenase.
Elsevier
